IL1A antibody (AA 113-271) (FITC)

Catalog No. ABIN1045778

The Rabbit Polyclonal anti-IL1A antibody has been validated for WB and ELISA. It is suitable to detect IL1A in samples from Human.

Quick Overview for IL1A antibody (AA 113-271) (FITC) (ABIN1045778)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1A antibody is conjugated to FITC

Application: Western Blotting (WB), ELISA

Product Details anti-IL1A Antibody (FITC)

Binding Specificity: AA 113-271

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Interleukin-1 alpha protein (113-271AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilutions:
ELISA: 1/200 - 1/1000 (using secondary ABIN547379).
Flow Cyt: Use at an assay dependant dilution.
IHC-Fr: 1/200 for staining of intracellular IL-1a.
Neut: 1/100. Incubate the sample with the antibody for at least 4 hours before testing. A contrl of similarly diluted normal rabbit IgG is recommended.
WB: 1/100 - 1/200 (using goat anti-rabbit (HRP) ABIN547379). Detects a band of approximately 17 kDa (mature) form as well as the 31 kDa (precursor) form in either non-denatured (native) or denatured samples.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid freeze and thaw cycles. Product is photosensitive and should be protected from light.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL1A

Background:

Background: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Aliases: BAF antibody, FAF antibody, Hematopoietin 1 antibody, Hematopoietin-1 antibody, IL 1 alpha antibody, IL 1A antibody, IL-1 alpha antibody, Il-1a antibody, IL1 ALPHA antibody, IL1 antibody, IL1A antibody, IL1A_HUMAN antibody, IL1F1 antibody, Interleukin 1 alpha antibody, Interleukin-1 alpha antibody, Interleukin1 alpha antibody, LAF antibody, LEM antibody, Preinterleukin 1 alpha antibody, Pro interleukin 1 alpha antibody

UniProt: P01583

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints