beta 2 Adrenergic Receptor antibody (Transmembrane Domain)

Catalog No. ABIN1048318

This Rabbit Polyclonal antibody specifically detects beta 2 Adrenergic Receptor in IHC and IHC (p). It exhibits reactivity toward Human and Monkey.

Quick Overview for beta 2 Adrenergic Receptor antibody (Transmembrane Domain) (ABIN1048318)

Target: beta 2 Adrenergic Receptor (ADRB2) (Adrenergic, beta-2-, Receptor, Surface (ADRB2))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta 2 Adrenergic Receptor antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-beta 2 Adrenergic Receptor Antibody

Binding Specificity: Transmembrane Domain

Brand: IHC-plus™

Specificity: Human ADRB2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey (100%) Pufferfish, Zebrafish, Stickleback (94%) Marmoset, Mouse, Rat, Hamster (88%) Bovine, Dog, Cat, Elephant, Pig, Guinea pig, Xenopus (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 5th transmembrane domain of human ADRB2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey (100%), Pufferfish, Zebrafish, Stickleback (94%), Marmoset, Mouse, Rat, Hamster (88%), Bovine, Dog, Cat, Elephant, Pig, Guinea pig, Xenopus (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (17 - 26 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for beta 2 Adrenergic Receptor

Target: beta 2 Adrenergic Receptor (ADRB2) (Adrenergic, beta-2-, Receptor, Surface (ADRB2))

Alternative Name: ADRB2

Background: Name/Gene ID: ADRB2
Subfamily: Adrenergic
Family: GPCR

Synonyms: ADRB2, ADRB2R, Adrenoceptor beta 2, surface, ADRBR, B2AR, Beta-2 adrenoceptor, Beta-2 adrenergic receptor, Beta-2 adrenoreceptor, BETA2AR, BAR, Adrenergic beta-2 receptor, Catecholamine receptor

Gene ID: 154

Pathways: cAMP Metabolic Process, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Brown Fat Cell Differentiation