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G Protein-Coupled Receptor 126 antibody (Internal Region)

GPR126 Reactivity: Human, Mouse, Rat IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1048706
  • Target See all G Protein-Coupled Receptor 126 (GPR126) Antibodies
    G Protein-Coupled Receptor 126 (GPR126)
    Binding Specificity
    • 15
    • 6
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 23
    • 17
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 34
    Rabbit
    Clonality
    • 34
    Polyclonal
    Conjugate
    • 20
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This G Protein-Coupled Receptor 126 antibody is un-conjugated
    Application
    • 15
    • 13
    • 13
    • 12
    • 8
    • 7
    • 5
    • 3
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human GPR126. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Mouse, Rat (100%) Gorilla, Marmoset, Rabbit (94%) Bovine (89%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 18 amino acid peptide from internal region of human GPR126. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Mouse, Rat (100%), Gorilla, Marmoset, Rabbit (94%), Bovine (89%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product GPR126 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (10 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    G Protein-Coupled Receptor 126 (GPR126)
    Alternative Name
    ADGRG6 / GPR126 (GPR126 Products)
    Synonyms
    GPR126 antibody, si:ch211-202p1.3 antibody, APG1 antibody, DREG antibody, PS1TP2 antibody, VIGR antibody, 1190004A11Rik antibody, AI449247 antibody, AW045736 antibody, Gm222 antibody, adhesion G protein-coupled receptor G6 antibody, ADGRG6 antibody, adgrg6 antibody, Adgrg6 antibody
    Background
    Name/Gene ID: ADGRG6
    Subfamily: Orphan-B
    Family: GPCR

    Synonyms: ADGRG6, APG1, G-protein coupled receptor 126, GPR126, PS1TP2, VIGR, DREG, G protein-coupled receptor 126
    Gene ID
    57211
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