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ADGRV1 / GPR98 antibody (N-Term)

ADGRV1 Reactivity: Human, Horse IHC, IHC (p), WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1048904
  • Target See all ADGRV1 / GPR98 (ADGRV1) products
    ADGRV1 / GPR98 (ADGRV1) (G-Protein Coupled Receptor 98 (ADGRV1))
    Binding Specificity
    N-Term
    Reactivity
    Human, Horse
    Host
    • 1
    Rabbit
    Clonality
    • 1
    Polyclonal
    Conjugate
    • 1
    This ADGRV1 / GPR98 antibody is un-conjugated
    Application
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Brand
    IHC-plus™
    Specificity
    Human GPR98. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Horse (100%) Monkey, Bat, Elephant (95%) Hamster (89%) Mouse (84%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 19 amino acid peptide from N-terminal extracellular domain of human GPR98. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Horse (100%), Monkey, Bat, Elephant (95%), Hamster (89%), Mouse (84%).

    Type of Immunogen: Synthetic peptide
  • Application Notes
    Approved: IHC, IHC-P (10 - 26 μg/mL), WB
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    ADGRV1 / GPR98 (ADGRV1) (G-Protein Coupled Receptor 98 (ADGRV1))
    Alternative Name
    ADGRV1 / GPR98 (ADGRV1 Products)
    Background
    Name/Gene ID: ADGRV1
    Subfamily: Orphan-A
    Family: GPCR

    Synonyms: ADGRV1, FEB4, KIAA0686, G-protein coupled receptor 98, MASS1, VLGR1b, Usher syndrome type-2C protein, USH2B, USH2C, G protein-coupled receptor 98, GPR98, KIAA1943, VLGR1
    Gene ID
    84059
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