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Metabotropic Glutamate Receptor 1 antibody (N-Term)

GRM1 Reactivity: Human, Cow, Rabbit IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1048925
  • Target See all Metabotropic Glutamate Receptor 1 (GRM1) Antibodies
    Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))
    Binding Specificity
    • 15
    • 15
    • 10
    • 7
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 63
    • 44
    • 42
    • 15
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Cow, Rabbit
    Host
    • 75
    • 16
    • 2
    • 1
    Rabbit
    Clonality
    • 71
    • 23
    Polyclonal
    Conjugate
    • 41
    • 7
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Metabotropic Glutamate Receptor 1 antibody is un-conjugated
    Application
    • 48
    • 30
    • 24
    • 24
    • 18
    • 15
    • 14
    • 14
    • 13
    • 8
    • 2
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human GRM1 / MGLUR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Elephant, Panda, Bovine, Rabbit (100%) Monkey, Marmoset, Mouse, Rat, Hamster, Dog, Bat, Horse, Opossum (94%) Chicken, Platypus (88%) Xenopus (82%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 17 amino acid peptide from N-terminal extracellular domain of human GRM1 / MGLUR1. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Elephant, Panda, Bovine, Rabbit (100%), Monkey, Marmoset, Mouse, Rat, Hamster, Dog, Bat, Horse, Opossum (94%), Chicken, Platypus (88%), Xenopus (82%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product GRM1 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (17 μg/mL)
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))
    Alternative Name
    GRM1 / MGLUR1 (GRM1 Products)
    Synonyms
    fj39d05 antibody, wu:fj39d05 antibody, si:ch211-51a19.1 antibody, Glura antibody, mGlutR antibody, GB18621 antibody, GPRC1A antibody, MGLU1 antibody, MGLUR1 antibody, SCAR13 antibody, 4930455H15Rik antibody, ENSMUSG00000075319 antibody, Gm10828 antibody, Gprc1a antibody, mGluR1 antibody, nmf373 antibody, rcw antibody, wobl antibody, glutamate metabotropic receptor 1 antibody, glutamate receptor, metabotropic 1a antibody, metabotropic glutamate receptor 1 antibody, glutamate receptor, metabotropic 1 antibody, GRM1 antibody, grm1a antibody, mGlutR1 antibody, Grm1 antibody
    Background
    Name/Gene ID: GRM1
    Subfamily: Metabotropic glutamate
    Family: GPCR

    Synonyms: GRM1, GRM1A, GPRC1A, MGlu1, MGLUR1, SCAR13, MGLUR1A
    Gene ID
    2911
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