GRPR antibody (Gastrin-Releasing Peptide Receptor) (Extracellular Domain)

Details for Product anti-GRPR Antibody No. ABIN1048947
Antigen
  • GRPR
  • GRP-R
  • gastrin releasing peptide receptor
  • GRPR
  • Grpr
Epitope
Extracellular Domain
15
10
7
4
3
2
2
2
2
1
1
1
1
1
Reactivity
Bat, Dog (Canine), Horse (Equine), Human, Monkey, Pig (Porcine), Rabbit
71
29
26
3
2
1
1
1
1
Host
Rabbit
71
2
2
Clonality
Polyclonal
Conjugate
This GRPR antibody is un-conjugated
5
4
3
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
48
36
30
15
9
7
4
2
1
1
Options
Brand IHC-plus™
Immunogen Synthetic 18 amino acid peptide from 2nd extracellular domain of human GRPR. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Horse, Rabbit, Pig (100%), Bovine (94%), Mouse, Rat, Hamster (83%).

Type of Immunogen: Synthetic peptide
Specificity Human GRPR. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Horse, Rabbit, Pig (100%) Bovine (94%) Mouse, Rat, Hamster (83%).
Purification Immunoaffinity purified
Plasmids, Primers & others Plasmids, Primers & others GRPR products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name GRPR (GRPR Antibody Abstract)
Background Name/Gene ID: GRPR
Subfamily: Bombesin
Family: GPCR

Synonyms: GRPR, GRP-R, Bombesin BB2 receptor, GRP receptor, Grp/bombesin receptor
Gene ID 2925
Application Notes Approved: IHC, IHC-P (4 μg/mL), WB
Comment

Target Species of Antibody: Human

Assay Procedure

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Gastrin-Releasing Peptide Receptor (GRPR) (Extracellular Domain) antibody (ABIN1048947) Anti-GRPR antibody ABIN1048947 IHC staining of human pancreas, islet of Langerhans. ...
Immunohistochemistry (IHC) image for anti-Gastrin-Releasing Peptide Receptor (GRPR) (Extracellular Domain) antibody (ABIN1048947) Anti-GRPR antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry ...
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