PTH1R antibody (N-Term)

Catalog No. ABIN1049266

This Rabbit Polyclonal antibody specifically detects PTH1R in IHC and IHC (p). It exhibits reactivity toward Human and Monkey.

Quick Overview for PTH1R antibody (N-Term) (ABIN1049266)

Target: PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTH1R antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PTH1R Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human PTH1R. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except UHRF1BP1 (40 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Monkey, Marmoset (100%) Gorilla, Gibbon, Bovine, Pig (95%) Orangutan, Elephant, Dog (90%) Panda, Horse (85%) Rabbit (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 20 amino acid peptide from N-terminal extracellular domain of human PTH1R. Percent identity with other species by BLAST analysis: Human, Monkey, Marmoset (100%), Gorilla, Gibbon, Bovine, Pig (95%), Orangutan, Elephant, Dog (90%), Panda, Horse (85%), Rabbit (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2 - 4 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PTH1R

Target: PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))

Alternative Name: PTHR

Background: Name/Gene ID: PTH1R
Subfamily: Parathyroid hormone
Family: GPCR

Synonyms: PTH1R, Parathyroid hormone 1 receptor, Parathyroid hormone receptor 1, PFE, Pth-related peptide receptor, PTHR, PTH/PTHr receptor, Pth/pthrp receptor, Parathyroid hormone receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTHR1, PTHrP-R, PTHrP-receptor

Gene ID: 5745

Pathways: Regulation of Carbohydrate Metabolic Process