TAAR1 antibody (Trace Amine Associated Receptor 1) (N-Term)

Details for Product anti-TAAR1 Antibody No. ABIN1049379
Antigen
Epitope
N-Term
8
7
5
4
3
2
2
1
1
1
Reactivity
Human, Monkey
51
21
13
11
3
Host
Rabbit
53
Clonality
Polyclonal
Conjugate
This TAAR1 antibody is un-conjugated
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
32
26
19
8
1
Options
Brand IHC-plus™
Immunogen Synthetic 16 amino acid peptide from N-terminus of human TAAR1. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Tamarin (100%), Orangutan, Cat, Horse (88%), Bovine, Elephant, Panda, Rabbit, Pig (81%).

Type of Immunogen: Synthetic peptide
Specificity Human TAAR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Tamarin (100%) Orangutan, Cat, Horse (88%) Bovine, Elephant, Panda, Rabbit, Pig (81%).
Purification Immunoaffinity purified
Antigen
Alternative Name TAAR1 / TA1 (TAAR1 Antibody Abstract)
Background Name/Gene ID: TAAR1
Subfamily: Trace amine
Family: GPCR

Synonyms: TAAR1, RP11-295F4.9, RTAR1, Snorf33, TRAR1, TaR-1, TAR1, TA1, Trace amine receptor 1
Gene ID 134864
Application Notes Approved: IHC, IHC-P (1.3 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months
Supplier Images
Image no. 1 for anti-Trace Amine Associated Receptor 1 (TAAR1) (N-Term) antibody (ABIN1049379) Anti-TAAR1 antibody ABIN1049379 IHC staining of human brain, amygdala. Immunohistoch...