USP20 antibody (Internal Region)

Catalog No. ABIN1049451

This anti-USP20 antibody is a Rabbit Polyclonal antibody detecting USP20 in IHC and IHC (p). Suitable for Human, Dog, Rabbit, Horse and Hamster.

Quick Overview for USP20 antibody (Internal Region) (ABIN1049451)

Target: USP20 (Ubiquitin Specific Peptidase 20 (USP20))

Reactivity: Human, Dog, Rabbit, Horse, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP20 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-USP20 Antibody

Binding Specificity: Internal Region

Brand: IHC-plus™

Specificity: Human USP20. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Hamster, Elephant, Dog, Horse, Rabbit (100%) Orangutan, Marmoset, Mouse, Rat, Panda, Bat, Bovine, Pig (94%) Opossum, Platypus (89%) Turkey, Chicken (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from internal region of human USP20. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Hamster, Elephant, Dog, Horse, Rabbit (100%), Orangutan, Marmoset, Mouse, Rat, Panda, Bat, Bovine, Pig (94%), Opossum, Platypus (89%), Turkey, Chicken (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for USP20

Target: USP20 (Ubiquitin Specific Peptidase 20 (USP20))

Alternative Name: VDU2 / USP20

Background: Name/Gene ID: USP20
Subfamily: Cysteine C19
Family: Protease

Synonyms: USP20, Deubiquitinating enzyme 20, Ubiquitin specific protease 20, Ubiquitin thioesterase 20, HVDU2, KIAA1003, LSFR3A, Ubiquitin thiolesterase 20, VDU2

Gene ID: 10868

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling