USP20 antibody (Middle Region)

Catalog No. ABIN2788022

The Rabbit Polyclonal anti-USP20 antibody is suitable to detect USP20 in samples from Human, Mouse, Rat, Dog, Rabbit, Horse, Cow and Guinea Pig. It has been validated for WB.

Quick Overview for USP20 antibody (Middle Region) (ABIN2788022)

Target: USP20 (Ubiquitin Specific Peptidase 20 (USP20))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Horse, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP20 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-USP20 Antibody

Binding Specificity: Middle Region

Purpose: USP20 Antibody - middle region

Sequence: PAEAQPPSPR SSSPCRTPEP DNDAHLRSSS RPCSPVHHHE GHAKLSSSPP

Predicted Reactivity: Cow: 92%, Dog: 92%, Guinea Pig: 100%, Horse: 92%, Human: 100%, Mouse: 85%, Rabbit: 100%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against USP20. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human USP20

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP59314-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for USP20

Target: USP20 (Ubiquitin Specific Peptidase 20 (USP20))

Alternative Name: USP20

Background:

Background Information: USP20 is a deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling.USP20 acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). USP20 plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. USP20 deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity.USP20 mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Gene Name: Ubiquitin specific peptidase 20

Alternative Symbols: VDU2, hVDU2, LSFR3A

Protein Name: Ubiquitin carboxyl-terminal hydrolase 20

Molecular Weight: 102kDa

Gene ID: 10868

NCBI Accession: NP_006667

UniProt: Q9Y2K6

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling