HIC2 antibody (AA 188-200)

Catalog No. ABIN1049504

The Goat Polyclonal anti-HIC2 antibody has been validated for WB and ELISA. It is suitable to detect HIC2 in samples from Human.

Quick Overview for HIC2 antibody (AA 188-200) (ABIN1049504)

Target: HIC2 (Hypermethylated in Cancer 2 (HIC2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HIC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-HIC2 Antibody

Binding Specificity: AA 188-200

Purpose: HIC2 (aa188-200)

Sequence: PQELPQAKGS DDE

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PQELPQAKGSDDE, from the internal region of the protein sequence according to NP_055909.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in lysates of cell line HepG2 (calculated MW of 66.2 kDa according to NP_055909.2). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HIC2

Target: HIC2 (Hypermethylated in Cancer 2 (HIC2))

Alternative Name: HIC2

Background: HIC2, hypermethylated in cancer 2, HRG22, ZBTB30, ZNF907, HIC1-related gene on chromosome 22 protein, hic-2, hic-3, hypermethylated in cancer 2 protein, zinc finger and BTB domain-containing protein 30

Gene ID: 23119, 58180, 287940

NCBI Accession: NP_055909