HIC2 antibody (Middle Region)

Catalog No. ABIN2777438

There is 1 publication for this product available. The Rabbit Polyclonal anti-HIC2 antibody is suitable to detect HIC2 in samples from Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig and Rabbit. It has been validated for WB and IF.

Quick Overview for HIC2 antibody (Middle Region) (ABIN2777438)

Target: HIC2 (Hypermethylated in Cancer 2 (HIC2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-HIC2 Antibody

Binding Specificity: Middle Region

Purpose: HIC2 Antibody - middle region

Sequence: CKEEEENGKD ASEDSAQSGS EGGSGHASAH YMYRQEGYET VSYGDNLYVC

Predicted Reactivity: Cow: 85%, Dog: 85%, Guinea Pig: 85%, Horse: 85%, Human: 100%, Mouse: 85%, Rabbit: 77%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against HIC2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HIC2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP31802-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-HIC2 antibody (ABIN2777438)

Collins, Wright, Edwards, Davis, Grinham, Cole, Goward, Aguado, Mallya, Mokrab, Huckle, Beare, Dunham: "A genome annotation-driven approach to cloning the human ORFeome." in: Genome biology, Vol. 5, Issue 10, pp. R84, (2004) (PubMed).

Target Details for HIC2

Target: HIC2 (Hypermethylated in Cancer 2 (HIC2))

Alternative Name: HIC2

Background:

Background Information: HIC2 contains 5 C2H2-type zinc fingers and 1 BTB (POZ) domain. It belongs to the krueppel C2H2-type zinc-finger protein family, Hic subfamily and is a transcriptional repressor.

Gene Name: Hypermethylated in cancer 2

Alternative Symbols: HRG22, ZBTB30, ZNF907

Protein Name: Hypermethylated in cancer 2 protein

Molecular Weight: 66kDa

Gene ID: 23119

NCBI Accession: NP_055909

UniProt: Q96JB3