CD34 antibody (C-Term)

Catalog No. ABIN1049505

This anti-CD34 antibody is a Goat Polyclonal antibody detecting CD34 in IF and ELISA. Suitable for Human.

Quick Overview for CD34 antibody (C-Term) (ABIN1049505)

Target: CD34

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Immunofluorescence (IF), ELISA

Grade: Verified

Product Details anti-CD34 Antibody

Binding Specificity: C-Term

Purpose: CD34-T

Sequence: SPTGERLELE P

Specificity: This antibody is expected to recognize reported isoform CD34-T (NP_001020280.1) only.

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SPTGERLELEP, from the of the protein sequence according to NP_001764.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary testing showed bands at approx 85+90 kDa in Human Peripheral Blood Mononucleocytes lysate (calculated MW of 35.0 kDa according to NP_001764.1). The observed MW correspond to glycosylated forms, routinely observed by other sources.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the plasma membranes and nuclei of Jurkat and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: A customer reported weak staining of Jurkat cel

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background: CD34, CD34 Molecule, CD34 antigen, OTTHUMP00000034733, OTTHUMP00000034734, hematopoietic progenitor cell antigen CD34, CD34-T

Gene ID: 947, 12490

NCBI Accession: NP_001764