C3 antibody (AA 681-693)

Catalog No. ABIN1049513

The Goat Polyclonal anti-C3 antibody has been validated for ELISA. It is suitable to detect C3 in samples from Human.

Quick Overview for C3 antibody (AA 681-693) (ABIN1049513)

Target: C3 (Complement Component 3 (C3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This C3 antibody is un-conjugated

Application: ELISA

Grade: Recent

Product Details anti-C3 Antibody

Binding Specificity: AA 681-693

Purpose: complement C3 (aa681-693)

Sequence: DKVGKYPKEL RKC

Specificity: This antibody is expected to recognize the C3 alpha chain and C3a anaphylatoxin,

Predicted Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence DKVGKYPKELRKC, from the internal region of the protein sequence according to NP_000055.2.

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Preliminary experiments gave an approx 125 kDa band in Human Liver lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104 kDa according to the largest fragment C3 alpha chain (aa672-1663). Although this fragment is glycosylated, we have not yet found support in the literature for the observed size. The 125 kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field. Please note that a different antibody developed to a different part of the protein showed identical results: EB11703.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for C3

Target: C3 (Complement Component 3 (C3))

Alternative Name: Complement Component 3

Background: C3, complement component 3, AHUS5, ARMD9, ASP, CPAMD1, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, OTTHUMP00000197086, acylation-stimulating protein cleavage product, complement C3, complement component C3

Gene ID: 718

NCBI Accession: NP_000055

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling