PPY antibody

Catalog No. ABIN105318

Supplier Product No.: 214-a01-118

This anti-PPY antibody is a Dog Polyclonal antibody detecting PPY in . Suitable for Pig.

Quick Overview for PPY antibody (ABIN105318)

Target: PPY (Pancreatic Polypeptide (PPY))

Reactivity: Pig

Host: Dog

Clonality: Polyclonal

Conjugate: This PPY antibody is un-conjugated

Application: Please inquire

Product Details anti-PPY Antibody

Supplier Product No.: 214-a01-118

Supplier: Rockland

Purpose: Pancreatic Islet Lysate Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog Serum.

Characteristics: Synonyms: IgG fraction of Anti-pancreatic islet lysate, Dog Anti-Pancreatic lysate (porcine pancreas), Anti-pancreatic lysate produced in dog, dog anti-Pancreatic Islet Lysate Antibody, canine anti-Pancreatic Islet Lysate Antibody

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Optional[Flow Cytometry Dilution]: This product has been assayed against 1.0 μg of Pancreatic Islet Lysate [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) code #604-1302 and o-Phenylenediamine (OPD) as a substrate.  A working dilution of 1:50 to 1:100 is suggested for this product.  This product has been assayed against 1.5 μg of Pancreatic Islet Lysate [Porcine Pancreas] per lane by immunoblot (western) under reducing conditions of SDS-PAGE prior to transfer to nitrocellulose and detection using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) code #604-1302 and Diaminobenzidine (DAB) as a substrate.  A working dilution of 1:50 to 1:200 is suggested for this product.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for PPY

Target: PPY (Pancreatic Polypeptide (PPY))

Alternative Name: PPY

Background: Background: Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions. It may be used as a marker of the viability of xenotransplanted fetal pancreatic tissue in the immediate post-transplant period.

UniProt: P01300

Pathways: Feeding Behaviour