Peroxidase antibody

Catalog No. ABIN105506

Supplier Product No.: 200-2138-0100

This anti-Peroxidase antibody is a Guinea Pig Polyclonal antibody detecting Peroxidase in WB, ELISA and IHC. Suitable for Horseradish.

Quick Overview for Peroxidase antibody (ABIN105506)

Target: Peroxidase

Reactivity: Horseradish

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This Peroxidase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-Peroxidase Antibody

Supplier Product No.: 200-2138-0100

Supplier: Rockland

Cross-Reactivity (Details): Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.

Purity: Anti-Peroxidase (Horseradish) (Guinea Pig) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Guinea Pig Serum as well as purified and partially purified Peroxidase [Horseradish].

Endotoxin Level: Low Endotoxin : No

Immunogen: Peroxidase [Horseradish]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Peroxidase (Horseradish) (Guinea Pig) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
ELISA Dilution: 1:20.000 - 1:100.000
Immunohistochemistry Dilution: 1:1.000 - 1:5.000
Western Blot Dilution: 1:2.000 - 1:10.000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Peroxidase

Target: Peroxidase

Background: Horse Radish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Peroxidase (Horseradish) (Guinea Pig) Antibody is ideal for investigators in Microbiology, Immunology, and Cell Biology research. Synonyms: HRP, Horseradish peroxidase, Guinea pig-anti-peroxidase antibody