SOD1 antibody

Catalog No. ABIN107158

Supplier Product No.: 200-4191-0100

This Rabbit Polyclonal antibody specifically detects SOD1 in WB and ELISA. It exhibits reactivity toward Human and Cow.

Quick Overview for SOD1 antibody (ABIN107158)

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reactivity: Human, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SOD1 Antibody

Supplier Product No.: 200-4191-0100

Supplier: Rockland

Purpose: Superoxide Dismutase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Superoxide Dismutase [Bovine erythrocytes].

Characteristics: Synonyms: rabbit anti-Superoxide Dismutase 1 Antibody, rabbit anti-SOD1 antibody, ALS 1 antibody, ALS antibody, ALS1 antibody, Amyotrophic lateral sclerosis 1 adult antibody, Amyotrophic lateral sclerosis 1 antibody, Cu/Zn SOD antibody, Cu/Zn superoxide dismutase antibody, Homodimer antibody, SOD 1

Purification: Anti-Superoxide Dismutase (SOD) is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Superoxide Dismutase [Bovine erythrocytes]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Superoxide Dismutase (SOD) Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Superoxide Dismutase [Bovine erythrocytes] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:50,000 is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for SOD1

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Alternative Name: SOD1

Background: Background: Superoxide Dismutase destroys radicals which are normally produced within the cells and which are toxic to biological systems.

Gene ID: 281495

UniProt: P00442

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis