PRICKLE4 antibody (N-Term)

Catalog No. ABIN1077371

The Rabbit Polyclonal anti-PRICKLE4 antibody is suitable to detect PRICKLE4 in samples from Human. It has been validated for ELISA, WB, IF and ICC.

Quick Overview for PRICKLE4 antibody (N-Term) (ABIN1077371)

Target: PRICKLE4 (Prickle Homolog 4 (PRICKLE4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRICKLE4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PRICKLE4 Antibody

Binding Specificity: N-Term

Specificity: Two isoforms of PRICKLE4 are known to exist. PRICKLE4 antibody is predicted to not cross-react with other PRICKLE protein family members.

Purification: Affinity chromatography purified via peptide column

Immunogen: PRICKLE4 antibody was raised against an AA 18 synthetic peptide near the amino terminus of human PRICKLE4.

Isotype: IgG

Application Details

Application Notes: PRICKLE4 antibody can be used for detection of PRICKLE4 by Western blot at 0.25 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL. Optimal dilutions for each application to be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: 4 °C/-20 °C

Storage Comment: PRICKLE4 antibody can be stored at 4 °C for three months and -20 °C, stable for up to one year.

Expiry Date: 12 months

Target Details for PRICKLE4

Target: PRICKLE4 (Prickle Homolog 4 (PRICKLE4))

Alternative Name: PRICKLE4

Background: Synonyms:
REST, NRSF-interacting LIM domain protein 4, OBTP, OEBT, TOMM6, C6orf49

PRICKLE4 is a member of the LIM family of proteins. It possesses two LIM domains as well as a PET domain and is thought to localize to the nucleus. Its expression in multiple malignant tissue differentiations indicate that it may play a role in cancer differentiation. Two other members of the LIM family, PRICKLE1 and PRICKLE2, are thought to function in the noncanonical WNT signaling pathway which regulates intracellular calcium release and planar cell polarity, suggesting the PRICKLE4 may play a similar role.

Gene ID: 29964

NCBI Accession: NP_037529