FAS antibody (AA 26-173)

Catalog No. ABIN1078008

This anti-FAS antibody is a Rabbit Polyclonal antibody detecting FAS in WB, IHC, IP and ICC. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for FAS antibody (AA 26-173) (ABIN1078008)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-FAS Antibody

Binding Specificity: AA 26-173

Purpose: Polyclonal Antibody to Factor Related Apoptosis (FAS)

Specificity: The antibody is a rabbit polyclonal antibody raised against FAS. It has been selected for its ability to recognize FAS in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Factor Related Apoptosis (FAS)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-FAS antibody (ABIN1078008)

Malik, Pal, Gupta: "EGF-mediated reduced miR-92a-1-5p controls HTR-8/SVneo cell invasion through activation of MAPK8 and FAS which in turn increase MMP-2/-9 expression." in: Scientific reports, Vol. 10, Issue 1, pp. 12274, (2020) (PubMed).

Garnero: "The Utility of Biomarkers in Osteoporosis Management." in: Molecular diagnosis & therapy, Vol. 21, Issue 4, pp. 401-418, (2017) (PubMed).

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: Factor Related Apoptosis

Background: CD95, ALPS1A, ALPS1-A, APO1, APT1, FAS1, FASTM, TNFRSF6, Fas Receptor, TNF Receptor Superfamily Member 6, Tumor Necrosis Factor Receptor Superfamily Member 6

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity