MBP antibody (AA 220-301)

Catalog No. ABIN1078364

There is 1 publication for this product available. The Rabbit Polyclonal anti-MBP antibody is suitable to detect MBP in samples from Human. It has been validated for WB, IHC, ICC and IP.

Quick Overview for MBP antibody (AA 220-301) (ABIN1078364)

Target: MBP (Myelin Basic Protein (MBP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-MBP Antibody

Binding Specificity: AA 220-301

Purpose: Polyclonal Antibody to Myelin Basic Protein (MBP)

Specificity: The antibody is a rabbit polyclonal antibody raised against MBP. It has been selected for its ability to recognize MBP in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Myelin Basic Protein (MBP) corresdonding to Val220~Met301 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-MBP antibody (ABIN1078364)

Bleyer, Curths, Dahlmann, Wichmann, Bauer, Moritz, Braun, Knauf, Kaup, Gruber-Dujardin: "Morphology and staining behavior of neutrophilic and eosinophilic granulocytes of the common marmoset (Callithrix jacchus)." in: Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, (2016) (PubMed).

Target Details for MBP

Target: MBP (Myelin Basic Protein (MBP))

Alternative Name: Myelin Basic Protein

Background: Myelin A1 protein, Myelin membrane encephalitogenic protein