Trypsin Inhibitor antibody

Catalog No. ABIN107866

Supplier Product No.: 200-4187-0100

The Rabbit Polyclonal anti-Trypsin Inhibitor antibody is suitable to detect Trypsin Inhibitor in samples from Lima bean. It has been validated for WB and ELISA.

Quick Overview for Trypsin Inhibitor antibody (ABIN107866)

Target: Trypsin Inhibitor

Reactivity: Lima bean

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Trypsin Inhibitor antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Trypsin Inhibitor Antibody

Supplier Product No.: 200-4187-0100

Supplier: Rockland

Purpose: Trypsin Inhibitor Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Lima Bean].

Characteristics: Synonyms: rabbit anti-trypsin inhibitor antibody, 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody

Purification: Anti-Trypsin Inhibitor is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Trypsin Inhibitor [Lima Bean]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Trypsin Inhibitor antibody has been assayed against 1.0 μg of Trypsin Inhibitor [Lima Bean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:15,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Trypsin Inhibitor

Target: Trypsin Inhibitor

Background: Background: Soy Bean Trypsin Inhibitor is a serine protease inhibitor, an inhibitor of trypsin, therefore preventing the further breakdown of proteins.

Gene ID: 547831

UniProt: P01070