XDH antibody

Catalog No. ABIN108315

Supplier Product No.: 200-4183-0100

This Rabbit Polyclonal antibody specifically detects XDH in WB, IP and ELISA. It exhibits reactivity toward Cow.

Quick Overview for XDH antibody (ABIN108315)

Target: XDH (Xanthine Dehydrogenase (XDH))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XDH antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Product Details anti-XDH Antibody

Supplier Product No.: 200-4183-0100

Supplier: Rockland

Purpose: Xanthine Oxidase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Xanthine Oxidase [Bovine Buttermilk].

Characteristics: Synonyms: rabbit anti-Xanthine Oxidase Antibody, Xanthine dehydrogenase antibody, Xanthine dehydrogenase/oxidase antibody, Xanthine oxidase antibody, Xanthine oxidoreductase antibody, xdh antibody, xdha antibody, XO antibody, xor antibody

Purification: Anti-Xanthine Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Xanthine Oxidase [Bovine Buttermilk]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Xanthine Oxidase antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Xanthine Oxidase [Bovine Buttermilk] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for XDH

Target: XDH (Xanthine Dehydrogenase (XDH))

Alternative Name: XDH

Background: Background: Xanthine dehydrogenase/oxidase is a key enzyme in purine degradation. It catalyzes the oxidation of hypoxanthine to xanthine. It catalyzes the oxidation of xanthine to uric acid. It contributes to the generation of reactive oxygen species. Xanthine dehydrogenase/oxidase can be converted from the dehydrogenase form (D) to the oxidase form (O) irreversibly by proteolysis or reversibly through the oxidation of sulfhydryl groups.

Gene ID: 280960

UniProt: P80457

Pathways: Positive Regulation of Endopeptidase Activity