GLUD1 antibody

Catalog No. ABIN108452

The Rabbit Polyclonal anti-GLUD1 antibody is suitable to detect GLUD1 in samples from Arabidopsis thaliana, Tomato and Lupinus luteus. It has been validated for WB and IL.

Quick Overview for GLUD1 antibody (ABIN108452)

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reactivity: Arabidopsis thaliana, Tomato, Lupinus luteus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLUD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunolocalization (IL)

Product Details anti-GLUD1 Antibody

Purpose: Anti-GDH1 | Glutamate dehydrogenase 1

Cross-Reactivity (Details): Arabidopsis thaliana, Lupinus luteus, Solanum lycopersicum
Not reactive in: Algae, Brassica rapa

Predicted Reactivity: Actinidia chinensis,Lactuca sativa,Nicotiana tabacum,Vitis vinifera

Purification: Serum

Immunogen: KLH-conjugated synthetic peptide derived from a full-length cDNA sequence of Lupinus luteus GDH1 Q5QDM6_LUPLU

Application Details

Application Notes: 1 : 1500 (IL), 1 : 5000-1 : 10 000 (WB)

Comment:

A number of degradation products may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and16kDa. D1 degradation is a complex set of events and the products observed canbe influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559,shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown product.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for GLUD1

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Alternative Name: GDH1

Background: Glutamate dehydrogenase 1 (EC=1.4.1.3) is an enzyme localized in mitochondrial matrix which plays a key role in nitrogen and glutamate metabolism.

Molecular Weight: 44 kDa

Gene ID: 831935

UniProt: Q5QDM6

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effect