GLUD1 antibody

Catalog No. ABIN108452

The Rabbit Polyclonal anti-GLUD1 antibody has been validated for WB and IL. It is suitable to detect GLUD1 in samples from Arabidopsis thaliana, Tomato and Lupinus luteus.

Quick Overview for GLUD1 antibody (ABIN108452)

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reactivity: Arabidopsis thaliana, Tomato, Lupinus luteus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLUD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunolocalization (IL)

Product Details anti-GLUD1 Antibody

Cross-Reactivity (Details): Not reactive in: algae

Predicted Reactivity: Actinidia chinensis, Lactuca sativa, Nicotiana tabacum, Vitis vinifera

Characteristics: Expected / apparent Molecular Weight of the Antigene: 44 kDa

Purification: serum

Immunogen: KLH-conjugated synthetic peptide derived from a full-length cDNA sequence of yellow lupine GDH1 Q5QDM6_LUPLU

Application Details

Application Notes: 1:5000 - 1:10 000 with standard ECL (WB), 1: 1500 (IL)

Comment:

A number of degradation products may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and16kDa. D1 degradation is a complex set of events and the products observed canbe influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559,shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown product.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for GLUD1

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Alternative Name: GDH1

Background: AGI Code: At5g18170
Glutamate dehydrogenase 1 (EC=1.4.1.3) is an enzyme localized in mitochondrial matrix which plays a key role in nitrogen and glutamate metabolism.

Molecular Weight: 44 kDa

UniProt: Q5QDM6

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effect