A2BP1 antibody (AA 394-646)

Catalog No. ABIN108467

There is 1 publication for this product available. The Rabbit Polyclonal anti-A2BP1 antibody is suitable to detect A2BP1 in samples from Chlamydomonas reinhardtii. It has been validated for WB and IF.

Quick Overview for A2BP1 antibody (AA 394-646) (ABIN108467)

Target: A2BP1 (Ataxin 2-Binding Protein 1 (A2BP1))

Reactivity: Chlamydomonas reinhardtii

Host: Rabbit

Clonality: Polyclonal

Conjugate: This A2BP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-A2BP1 Antibody

Binding Specificity: AA 394-646

Purpose: Anti-FOX1 | plasma membrane ferroxidase FLP1

Cross-Reactivity (Details): Chlamydomonas reinhardtii
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Chlamydomonas reinhardtii

Purification: Serum

Immunogen: residues 394-646 from Chlamydomonas reinhardtii FOX1 A8IZT9 fused to TrxA

Application Details

Application Notes: 1 : 1000-1 : 5000 (WB)

Comment:

This antibody has been used on Chlamydomonas cells expressing endogenous Fox1 and on HEK293 cells transiently expressing Fox1. Cells were resuspended in PBS plated on poly-lysine coated coverslips, fixed in 3.7% formaldehyde. Blocked in 3% BSA in PBS. 1:500 dilution of anti-FOX1 in 3% BSA. Secondary antibody was anti-rabbit Alexa fluor 488 1:300.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-A2BP1 antibody (ABIN108467)

Terauchi, Peers, Kobayashi, Niyogi, Merchant: "Trophic status of Chlamydomonas reinhardtii influences the impact of iron deficiency on photosynthesis." in: Photosynthesis research, Vol. 105, Issue 1, pp. 39-49, (2010) (PubMed).

Target Details for A2BP1

Target: A2BP1 (Ataxin 2-Binding Protein 1 (A2BP1))

Alternative Name: FOX1

Background:

Background: A copper-containing protein in Chlamydomonas reinhardtii is a plasma membrane ferroxidase, encoded by the FOX1 gene, involved in Fe assimilation. FOX1 is most highly related to the mammalian homologues hephaestin and ceruloplasmin, its occurrence and pattern of expression in Chlamydomonas indicate a role for copper in iron assimilation in a photosynthetic species.

Additional information: FOX1 is 10-20-fold induced in iron-deficiency and its occurrence therefore serves as a marker of iron nutrition state in Chlamydomonas. For detection image please refer to the article below.

Molecular Weight: 122 | 120 kDa

Gene ID: 394646

UniProt: A8IZT9