A2BP1 antibody (AA 394-646)

Catalog No. ABIN108467

Product Details anti-A2BP1 Antibody

Target: A2BP1 (Ataxin 2-Binding Protein 1 (A2BP1))

Binding Specificity: AA 394-646

Reactivity: Chlamydomonas reinhardtii

Host: Rabbit

Clonality: Polyclonal

Conjugate: This A2BP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: Chlamydomonas reinhardtii

Characteristics: Expected / apparent Molecular Weight of the Antigene: 122 / 120 kDa

Purification: serum

Immunogen: residues 394-646 from Chlamydomonas reinhardtii FOX1 A8IZT9 fused to TrxA

Application Details

Application Notes: 1: 1000 - 1: 5000 with standard ECL (WB),

Comment:

This antibody has been used on Chlamydomonas cells expressing endogenous Fox1 and on HEK293 cells transiently expressing Fox1. Cells were resuspended in PBS plated on poly-lysine coated coverslips, fixed in 3.7% formaldehyde. Blocked in 3% BSA in PBS. 1:500 dilution of anti-FOX1 in 3% BSA. Secondary antibody was anti-rabbit Alexa fluor 488 1:300.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References for anti-A2BP1 antibody (ABIN108467)

Terauchi, Peers, Kobayashi, Niyogi, Merchant: "Trophic status of Chlamydomonas reinhardtii influences the impact of iron deficiency on photosynthesis." in: Photosynthesis research, Vol. 105, Issue 1, pp. 39-49, (2010) (PubMed).

Target Details for A2BP1

Target: A2BP1 (Ataxin 2-Binding Protein 1 (A2BP1))

Alternative Name: FOX1 (A2BP1 Products)

Background: A copper-containing protein in Chlamydomonas reinhardtii is a plasma membrane ferroxidase, encoded by the FOX1 gene, involved in Fe assimilation. FOX1 is most highly related to the mammalian homologues hephaestin and ceruloplasmin, its occurrence and pattern of expression in Chlamydomonas indicate a role for copper in iron assimilation in a photosynthetic species.

Molecular Weight: expected: 122 kDa, apparent: 120 kDa

Gene ID: 394646

UniProt: A8IZT9