PABP antibody

Catalog No. ABIN108538

This Mouse Monoclonal antibody specifically detects PABP in WB, ELISA, IF, IP, FACS and ICC. It exhibits reactivity toward Human, Rabbit, Chicken, Xenopus laevis and Hamster and has been mentioned in 3+ publications.

Quick Overview for PABP antibody (ABIN108538)

Target: PABP (PABPC1) (Poly(A) Binding Protein, Cytoplasmic 1 (PABPC1))

Reactivity: Human, Rabbit, Chicken, Xenopus laevis, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This PABP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 10E10

Product Details anti-PABP Antibody

Purification: Protein G

Immunogen: Recombinant PABP (Human) expressed from its 1.85 kbp cDNA, NcoI to SspI

Isotype: IgG2b

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however try:WB: Use at an assay dependent dilution. Detects a band of approximately 70 kDa (predicted molecular weight: 71 kDa)ICC/IF: Use at an assay dependent dilution. PubMed: 17977970Flow Cyt: Use 1 μg for 106 cells

Comment:

Myeloma, fusion partners: SP2/0

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody (from supernatant) containing PBS + 0.1 % sodium azide,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-PABP antibody (ABIN108538)

Gorgoni, Richardson, Burgess, Anderson, Wilkie, Gautier, Martins, Brook, Sheets, Gray: "Poly(A)-binding proteins are functionally distinct and have essential roles during vertebrate development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 19, pp. 7844-9, (2011) (PubMed).

Unsworth, Raguz, Edwards, Higgins, Yagüe: "mRNA escape from stress granule sequestration is dictated by localization to the endoplasmic reticulum." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 9, pp. 3370-80, (2010) (PubMed).

Görlach, Burd, Dreyfuss: "The mRNA poly(A)-binding protein: localization, abundance, and RNA-binding specificity." in: Experimental cell research, Vol. 211, Issue 2, pp. 400-7, (1994) (PubMed).

Target Details for PABP

Target: PABP (PABPC1) (Poly(A) Binding Protein, Cytoplasmic 1 (PABPC1))

Alternative Name: PABP

Background: Synonyms:
PAB 1 antibody,PAB1 antibody,PABP 1 antibody,PABP1 antibody,PABPC 1 antibody,PABPC1antibody,PABPC2 antibody,PABPL1 antibody,Poly A binding protein 1 antibody,Poly A binding protein cytoplasmic1 antibody,Polyadenylate binding protein 1 antibody

Gene ID: 117282

OMIM: 604679

UniProt: P22626

Pathways: SARS-CoV-2 Protein Interactome