THO Complex 4 antibody

Catalog No. ABIN108544

This Mouse Monoclonal antibody specifically detects THO Complex 4 in WB, ELISA, IF, IP, IHC (p), ICC, FACS, ChIP and EM. It exhibits reactivity toward Human and Xenopus laevis and has been mentioned in 8+ publications.

Quick Overview for THO Complex 4 antibody (ABIN108544)

Target: THO Complex 4 (THOC4)

Reactivity: Human, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This THO Complex 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Chromatin Immunoprecipitation (ChIP), Electron Microscopy (EM)

Clone: 11G5

Product Details anti-THO Complex 4 Antibody

Purification: Protein A

Immunogen: Full length recombinant human ALY / REF fusion protein

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try:IHC-P: Use a concentration of 1 μg/mL.ICC/IF: 1/50. PubMed: 17103222WB: 1/1500. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa).

Comment:

Myeloma, fusion partners: SP2/0

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody (from supernatant) containing PBS + 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-THO Complex 4 antibody (ABIN108544)

Ding, Enriquez-Algeciras, Dave, Perez-Pinzon, Bhattacharya: "The role of deimination in ATP5b mRNA transport in a transgenic mouse model of multiple sclerosis." in: EMBO reports, Vol. 13, Issue 3, pp. 230-6, (2012) (PubMed).

Domínguez-Sánchez, Sáez, Japón, Aguilera, Luna: "Differential expression of THOC1 and ALY mRNP biogenesis/export factors in human cancers." in: BMC cancer, Vol. 11, pp. 77, (2011) (PubMed).

Thomas, Lischka, Müller, Stamminger: "The cellular DExD/H-box RNA-helicases UAP56 and URH49 exhibit a CRM1-independent nucleocytoplasmic shuttling activity." in: PLoS ONE, Vol. 6, Issue 7, pp. e22671, (2011) (PubMed).

Read, Digard: "Individual influenza A virus mRNAs show differential dependence on cellular NXF1/TAP for their nuclear export." in: The Journal of general virology, Vol. 91, Issue Pt 5, pp. 1290-301, (2010) (PubMed).

Katahira, Inoue, Hurt, Yoneda: "Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA." in: The EMBO journal, Vol. 28, Issue 5, pp. 556-67, (2009) (PubMed).

Nojima, Hirose, Kimura, Hagiwara: "The interaction between cap-binding complex and RNA export factor is required for intronless mRNA export." in: The Journal of biological chemistry, Vol. 282, Issue 21, pp. 15645-51, (2007) (PubMed).

van Koningsbruggen, Straasheijm, Sterrenburg, de Graaf, Dauwerse, Frants, van der Maarel: "FRG1P-mediated aggregation of proteins involved in pre-mRNA processing." in: Chromosoma, Vol. 116, Issue 1, pp. 53-64, (2007) (PubMed).

Kim, Yong, Kataoka, Abel, Diem, Dreyfuss: "The Y14 protein communicates to the cytoplasm the position of exon-exon junctions." in: The EMBO journal, Vol. 20, Issue 8, pp. 2062-8, (2001) (PubMed).

Target Details for THO Complex 4

Target: THO Complex 4 (THOC4)

Alternative Name: ALY

Background: Synonyms:
Ally of AML1 and LEF1 antibody,BEF antibody,bZIP enhancing factor antibody,REF antibody,THO complex 4antibody,THOC 4 antibody,THOC-4 antibody,THOC4 antibody,Transcriptional coactivatorantibody

Gene ID: 10189, 21681

OMIM: 604171