CENPE antibody

Catalog No. ABIN108563

This Mouse Monoclonal antibody specifically detects CENPE in IP, ICC, IF and FACS. It exhibits reactivity toward Human and has been mentioned in 10+ publications.

Quick Overview for CENPE antibody (ABIN108563)

Target: CENPE (Centromere Protein E, 312kDa (CENPE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CENPE antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 1H12

Product Details anti-CENPE Antibody

Purification: Affinity purified by ion exchange chromatography

Immunogen: Recombinant full length CENPE protein (human)

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try IP: Use at μg/mg of lysate. Use at a concentration of 3 μg/mL per 300 μg of lysate WB: Use a concentration of 0.5 - 1 μg/mL. Predicted molecular weight: 312 kDa. Only suitable for WB if IP is performed first Flow CytFlow Cyt: Use 1 μg for 106 cells

Comment:

Myeloma, fusion partners: SP2/0

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody (from supernatant) containing PBS 0.1 % sodium azide,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-CENPE antibody (ABIN108563)

Hu, Coughlin, Mitchison: "Midbody assembly and its regulation during cytokinesis." in: Molecular biology of the cell, Vol. 23, Issue 6, pp. 1024-34, (2012) (PubMed).

Serio, Margaria, Jensen, Oldani, Bartek, Bussolino, Lanzetti: "Small GTPase Rab5 participates in chromosome congression and regulates localization of the centromere-associated protein CENP-F to kinetochores." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 42, pp. 17337-42, (2011) (PubMed).

Kim, Holland, Lan, Cleveland: "Aurora kinases and protein phosphatase 1 mediate chromosome congression through regulation of CENP-E." in: Cell, Vol. 142, Issue 3, pp. 444-55, (2010) (PubMed).

Stimpson, Song, Jauch, Holtgreve-Grez, Hayden, Bridger, Sullivan: "Telomere disruption results in non-random formation of de novo dicentric chromosomes involving acrocentric human chromosomes." in: PLoS genetics, Vol. 6, Issue 8, (2010) (PubMed).

Townsend, Mason, Blackford, Miller, Chapman, Sedgwick, Barone, Turnell, Stewart: "Mediator of DNA damage checkpoint 1 (MDC1) regulates mitotic progression." in: The Journal of biological chemistry, Vol. 284, Issue 49, pp. 33939-48, (2009) (PubMed).

Cheng, Hsiao, Lin, Yu, Hsu, Chang, Lee, Huang, Howng, Hong: "Glycogen synthase kinase 3beta interacts with and phosphorylates the spindle-associated protein astrin." in: The Journal of biological chemistry, Vol. 283, Issue 4, pp. 2454-64, (2008) (PubMed).

Díaz-Martínez, Giménez-Abián, Azuma, Guacci, Giménez-Martín, Lanier, Clarke: "PIASgamma is required for faithful chromosome segregation in human cells." in: PLoS ONE, Vol. 1, pp. e53, (2007) (PubMed).

Schafer-Hales, Iaconelli, Snyder, Prussia, Nettles, El-Naggar, Khuri, Giannakakou, Marcus: "Farnesyl transferase inhibitors impair chromosomal maintenance in cell lines and human tumors by compromising CENP-E and CENP-F function." in: Molecular cancer therapeutics, Vol. 6, Issue 4, pp. 1317-28, (2007) (PubMed).

Chang, Coughlin, Mitchison: "Tankyrase-1 polymerization of poly(ADP-ribose) is required for spindle structure and function." in: Nature cell biology, Vol. 7, Issue 11, pp. 1133-9, (2005) (PubMed).

Yen, Compton, Wise, Zinkowski, Brinkley, Earnshaw, Cleveland: "CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase." in: The EMBO journal, Vol. 10, Issue 5, pp. 1245-54, (1991) (PubMed).

Target Details for CENPE

Target: CENPE (Centromere Protein E, 312kDa (CENPE))

Alternative Name: CENPE

Background: Synonyms:
CENP E antibody,Centromere autoantigen E antibody,Centromere protein E 312kDa antibody,Centromereprotein E antibody,Centromeric protein E antibody,KIF10 antibody,Kinesin family member 10 antibody,Kinesinrelated protein antibody

Gene ID: 1062, 296137

OMIM: 117143

UniProt: O08901

Pathways: M Phase, Maintenance of Protein Location