HNRNPU antibody

Catalog No. ABIN108577

This Mouse Monoclonal antibody specifically detects HNRNPU in WB, ELISA, IP, IF, IHC (p), ICC and FACS. It exhibits reactivity toward Human, Mouse and Monkey and has been mentioned in 8+ publications.

Quick Overview for HNRNPU antibody (ABIN108577)

Target: HNRNPU (Heterogeneous Nuclear Ribonucleoprotein U (Scaffold Attachment Factor A) (HNRNPU))

Reactivity: Human, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This HNRNPU antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 3G6

Product Details anti-HNRNPU Antibody

No Cross-Reactivity: Chicken, Lizard

Cross-Reactivity (Details): Mouse, Human, Monkey. Does not react with Chicken, Lizard

Purification: Protein A

Immunogen: RNPs eluted from an oligo (dt) cellulose column

Isotype: IgG1

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try,WB: Use at an assay dependent dilution. Detects a band of approximately 97 kDa (predicted molecular weight: 120 kDa).ICC/IFICC/IF: 1/100.Flow CytFlow Cyt: Use 1 μg for 106 cells.

Comment:

Myeloma, fusion partners: SP2/0

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody (from supernatant) containing PBS 0.1 % sodium azide,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-HNRNPU antibody (ABIN108577)

Ma, Matsunaga, Morimoto, Sakashita, Urano, Uchiyama, Fukui: "The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles." in: Journal of cell science, Vol. 124, Issue Pt 3, pp. 394-404, (2011) (PubMed).

Pare, López-Orozco, Hobman: "MicroRNA-binding is required for recruitment of human Argonaute 2 to stress granules and P-bodies." in: Biochemical and biophysical research communications, Vol. 414, Issue 1, pp. 259-64, (2011) (PubMed).

Raju, Fukuda, López-Iglesias, Göritz, Visa, Percipalle: "In neurons, activity-dependent association of dendritically transported mRNA transcripts with the transacting factor CBF-A is mediated by A2RE/RTS elements." in: Molecular biology of the cell, Vol. 22, Issue 11, pp. 1864-77, (2011) (PubMed).

Tserel, Runnel, Kisand, Pihlap, Bakhoff, Kolde, Peterson, Vilo, Peterson, Rebane: "MicroRNA expression profiles of human blood monocyte-derived dendritic cells and macrophages reveal miR-511 as putative positive regulator of Toll-like receptor 4." in: The Journal of biological chemistry, Vol. 286, Issue 30, pp. 26487-95, (2011) (PubMed).

Hadian, Vincendeau, Mäusbacher, Nagel, Hauck, Ueffing, Loyter, Werner, Wolff, Brack-Werner: "Identification of a heterogeneous nuclear ribonucleoprotein-recognition region in the HIV Rev protein." in: The Journal of biological chemistry, Vol. 284, Issue 48, pp. 33384-91, (2009) (PubMed).

Uchimura, Ichimura, Uwada, Tachibana, Sugahara, Nakao, Saitoh: "Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation." in: The Journal of biological chemistry, Vol. 281, Issue 32, pp. 23180-90, (2006) (PubMed).

Ahmad, Lingrel: "Kruppel-like factor 2 transcriptional regulation involves heterogeneous nuclear ribonucleoproteins and acetyltransferases." in: Biochemistry, Vol. 44, Issue 16, pp. 6276-85, (2005) (PubMed).

Dreyfuss, Choi, Adam: "Characterization of heterogeneous nuclear RNA-protein complexes in vivo with monoclonal antibodies." in: Molecular and cellular biology, Vol. 4, Issue 6, pp. 1104-14, (1984) (PubMed).

Target Details for HNRNPU

Target: HNRNPU (Heterogeneous Nuclear Ribonucleoprotein U (Scaffold Attachment Factor A) (HNRNPU))

Alternative Name: hnRNP U/p120

Background: Synonyms:
Heterogeneous nuclear ribonucleoprotein U antibody,hnRNP U protein antibody,HNRNPU antibody,hnRNPUprotein antibody,HNRPU antibody,p120 antibody,p120 nuclear protein antibody,pp120 antibody,SAF Aantibody,SAF-A antibody,SAFA antibody,Scaffold attachment factor A antibody,U21.1antibody

Gene ID: 3192, 51810

OMIM: 602869

UniProt: P31943