Zona Radiata Protein antibody

Catalog No. ABIN108744

Product Details

Target: Zona Radiata Protein

Reactivity: Salmon

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Specificity: O-146 binds with high affinity to the three zona radiata proteins alpha, beta and gamma in plasma samples from Atlantic salmon. The antibody cross-reacts well with Zrp in plasma samples from brown trout (Salmo trutta), rainbow trout (Oncorhynchus mykiss) and Arctic char (Salvelinus alpinus), reasonably well with Zrp from Atlantic cod (Gadus morhua), turbot (Scophthalmus maximus), flounder (Platichthys flesus) and halibut (Hippoglossus hippoglossus). Crossreactivity with zona pellucida proteins may be observed in fish ovaries or eggs. The degree of crossreactivity may differs between the different Zrp proteins and with the different methods employed. (Oncorhynchus mykiss), Arctic charr (Salvelinus alpinus), turbot (Scophthalmus maximus) and cod (Gadus morhua). The degree of cross-reactivity differs between species and with the methods employed.

Immunogen: Immunogen source: Eggshell proteins (zona radiata proteins and small amounts of zona pellucida proteins) purified from plasma of 17ß-estradiol treated Atlantic salmon.

Application Details

Application Notes: O-146 is well suited for ELISA and immunoblotting. Since assay conditions vary, the optimum dilution should be determined for each particular application. Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating. Normal dilution range: 1:100 - 1: 1000 (Salmoniformes) 1:50 - 1: 200 (Other species)

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C

Target Details

Target: Zona Radiata Protein

Synonyms: zrp antibody, zona radiata protein antibody, zrp antibody

Background: This product consists of Protein-G affinity-purified polyclonal rabbit antibodies against zona radiata proteins (Zrp) from Atlantic salmon (Salmo salar). The antibodies are dissolved in 0.1M Na-phosphate, 0.15M NaCl, 1% BSA, 0.1% Na-azide, pH 7.4.