CD40 Ligand antibody

Catalog No. ABIN1100607

This anti-CD40 Ligand antibody is a Goat Polyclonal antibody detecting CD40 Ligand in WB, IHC, ELISA and IHC (p). Suitable for Human.

Quick Overview for CD40 Ligand antibody (ABIN1100607)

Target: CD40 Ligand (CD40LG)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD40 Ligand antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD40 Ligand Antibody

Brand: IHC-plus™

Specificity: Human sCD40 Ligand

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human sCD40 Ligand.

Type of Immunogen: Cell extract

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7421 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. ELISA: To detect hsCD40-Ligand by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sCD40-Ligand (LS-C104498) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsCD40-Ligand. Western Blot: To detect hsCD40-Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40-Ligand is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40LG / CD40L / CD154

Background: Name/Gene ID: CD40LG
Family: TNF

Synonyms: CD40LG, CD154, CD154 antigen, CD40-L, CD40 ligand, CD40L, gp39, HIGM1, IGM, IMD3, T-BAM, TNFSF5, CD40 antigen ligand, CD54, HCD40L, T-B cell-activating molecule, T-cell antigen Gp39, TNF-related activation protein

Gene ID: 959

UniProt: P29965

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints