DBH antibody (AA 596-612)

Catalog No. ABIN1101082

This Sheep Polyclonal antibody specifically detects DBH in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for DBH antibody (AA 596-612) (ABIN1101082)

Target: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This DBH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-DBH Antibody

Binding Specificity: AA 596-612

Specificity: Dopamine Beta Hydroxylase (DbH) C-terminal. By immunoblot the antibody specifically reacts with a single (or double) band (s) of MW >70-75000 in samples of SDS-solubilized, DTT-reduced human adrenal medulla subjected to SDS-PAGE. Species Reactivity: By immunoblot: human, monkey, bovine and rabbit (slight).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon (94%) Monkey (88%) Horse, Rabbit (82%).

Purification: Affinity purified

Immunogen: Synthetic peptide, CPTSQGRSPAGPTVVSI-amide, from C-terminal region of human (DbH). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon (94%), Monkey (88%), Horse, Rabbit (82%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC (1:125 - 1:500), WB (1:125 - 1:500)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:125-1:500 on human, monkey and bovine adrenal medulla. Immunohistochemistry: 1:125-1:500, staining was evaluated in the locus ceruleus of formaldehyde-fixed, cryopreserved human and monkey brain. Showed strong staining on both human and monkey.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 0.09 % sodium azide, 0.1 mg/mL BSA, 50 % glycerol, 150  mM sodium chloride

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for DBH

Target: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Alternative Name: DBH/Dopamine Beta Hydroxylase

Background: Name/Gene ID: DBH
Family: Monooxygenase

Synonyms: DBH, Dopamine beta-monooxygenase, DBM, Dopamine beta-hydroxylase

Gene ID: 1621

UniProt: P09172

Pathways: Carbohydrate Homeostasis