FAP antibody (Internal Region)

Catalog No. ABIN1101478

This Rabbit Polyclonal antibody specifically detects FAP in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for FAP antibody (Internal Region) (ABIN1101478)

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FAP Antibody

Binding Specificity: Internal Region

Specificity: This antibody is known to react with mouse FAP protein. A BLAST analysis was used to suggest partial cross-reactivity with FAP from human and rat sources based on ~88 % homology with the immunizing sequence. Reactivity with FAP from other sources has not been determined.

Purification: Immunoaffinity purified

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse FAP protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB (1:500 - 1:2000)

Usage: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 88 kD in size corresponding to FAP by western blotting in the appropriate cell lysate or extract.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for FAP

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Alternative Name: FAP alpha

Background: Name/Gene ID: FAP
Subfamily: Serine S9
Family: Protease

Synonyms: FAP, DPPIV, FAP alpha, Fibroblast activation protein, FAPA, Seprase

Gene ID: 2191

UniProt: Q12884

Pathways: Tube Formation