LINE-1 antibody

Catalog No. ABIN1102848

The Chicken Polyclonal anti-LINE-1 antibody (ABIN1102848) specifically detects LINE-1 in ELISA and WB. The antibody is reactive with Human samples.

Quick Overview for LINE-1 antibody (ABIN1102848)

Target: LINE-1

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB)

Product Details

Specificity: Reactivity occurs against human L1/ORF2 protein and is useful in determining its presence in various assays. A BLAST analysis was used to suggest cross reactivity with L1/ORF2 proteins from chimpanzee sources based on 100 % homology with the immunizing sequences, and from macaque, fruit fly, cattle, dog, opossum, and rat sources based on 69 - 88 % homology with the immunizing sequences.

Purification: Protein A purified

Sterility: Sterile filtered

Immunogen: This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.

Type of Immunogen: Synthetic peptide

Isotype: IgY

Application Details

Application Notes: Approved: ELISA, WB (1:2000 - 1:10000)

Usage: This IgY fraction antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 149 kD in size corresponding to L1 protein by western blotting in the appropriate cell lysate or extract.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide, sterile filtered

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details

Target: LINE-1