Nestin antibody

Catalog No. ABIN1103237

The Mouse Monoclonal anti-Nestin antibody has been validated for IHC, WB, IHC (p) and IHC (fro). It is suitable to detect Nestin in samples from Human.

Quick Overview for Nestin antibody (ABIN1103237)

Target: Nestin (NES)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 10C2

Product Details anti-Nestin Antibody

Brand: IHC-plus™

Purification: Affinity purified

Immunogen: Human NES

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-Fr, IHC-P (20 μg/mL), WB

Usage: This 10C2 antibody has been tested by immunoblot analysis of cell line. This can be used at less than or equal to 5 μg per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freezing and store undiluted.

Storage: 4 °C

Storage Comment: Store at 4°C, avoid freeze-thaw cycles.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin

Background: Name/Gene ID: NES

Synonyms: NES, Nestin, Nbla00170

Gene ID: 10763

UniProt: P48681