TGFA antibody

Catalog No. ABIN1104644

The Rabbit Polyclonal anti-TGFA antibody is suitable to detect TGFA in samples from Human. It has been validated for IHC, WB, ELISA, IHC (p) and Neut.

Quick Overview for TGFA antibody (ABIN1104644)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TGFA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)

Product Details anti-TGFA Antibody

Specificity: Human TGF-alpha

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TGF-alpha.

Type of Immunogen: Cell extract

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (2.5 - 3.7 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7423 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human TGF-alpha (0.5 ng/mL), a concentration of 2.5-3.7 μg/mL of this antibody is required. ELISA: To detect Human TGF-alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TGF-alpha (LS-C104603) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TGF-alpha. Western Blot: To detect Human TGF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TGF-alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: TGFA / TGF Alpha

Background: Name/Gene ID: TGFA
Family: TGF beta

Synonyms: TGFA, Etgf, TFGA, TGF Alpha, Tgf type 1, TGF-alpha, Egf-like tgf

Gene ID: 7039

UniProt: P01135

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway