14-3-3 Pan antibody

Catalog No. ABIN1105134

Product Details

Target: 14-3-3 Pan

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Protein A agarose

Immunogen: Recombinant human 14-3-3beta

Clone: 3C8

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 50 % glycerol, pH 7.2, containing no preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C.

Target Details

Target: 14-3-3 Pan

Alternative Name: 14-3-3 Protein Pan

Background: 14-3-3 was first described as an abundant acidic protein in brain composing 1 % of the total soluble protein. The 14-3-3 family of proteins are expressed in all eukaryotic cells. There are at least seven highly conserved isoforms encoded by different gene products. These proteins have molecular weights of 29,000-32,000 and bind numerous cytoplasmic and nuclear signaling molecules including signaling molecules such as Raf, protein kinase C, p130Cas, BAD, and phosphatidylinositol 3-kinase. Interaction of 14-3-3 proteins with signaling molecules can localize, activate, inhibit, or stabilize the target molecules. Because 14-3-3 proteins are homo- and heterodimers, they have been considered as adaptor proteins that recruit and regulate the function of signaling molecules. They have been implicated in regulation of cell proliferation, cell cycle progression, apoptosis, and differentiation.