14-3-3 Pan (AA 174-245) antibody

Catalog No. ABIN726710

The Rabbit Polyclonal anti-14-3-3 Pan antibody is suitable to detect 14-3-3 Pan in samples from Human. It has been validated for WB, FACS, IHC (fro), IHC (p), IF (cc) and IF (p).

Quick Overview for 14-3-3 Pan (AA 174-245) antibody (ABIN726710)

Target: 14-3-3 Pan

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details

Binding Specificity: AA 174-245

Purpose: 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Antibody

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Sheep,Fruit Fly,yeast

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human 14-3-3

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: 14-3-3 Pan

Alternative Name: 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon

Background:

Synonyms: HS1, GW128, YWHAA, KCIP-1, HEL-S-1, 14-3-3 protein beta/alpha, Protein 154, Protein kinase C inhibitor protein 1, YWHAB

Background: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.

Gene ID: 7529

UniProt: P31946