Actin antibody (C-Term, pan)

Catalog No. ABIN1105234

This Mouse Monoclonal antibody specifically detects Actin in WB, IF and IHC (fro). It exhibits reactivity toward Human, Rat, Mouse, Chicken and Snake.

Quick Overview for Actin antibody (C-Term, pan) (ABIN1105234)

Target: Actin

Reactivity: Human, Rat, Mouse, Chicken, Snake

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: AC-40

Product Details anti-Actin Antibody

Binding Specificity: C-Term, pan

Purification: Goat anti-Mouse IgG Affinity Chromatography

Immunogen: Synthetic Actin C-terminal peptide Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.2 % Sodium Acetate or neutral PBS.

Concentration: 0.1 mg/mL

Buffer: 1.2 % Sodium Acetate containing 2 mg BSA and 0.01 mg Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at -20 °C. After reconstituion store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Actin

Target: Actin

Background: Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90 % sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin,two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins,this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.