nectin-3 antibody (Extracellular Domain)

Catalog No. ABIN1105755

The Rat Monoclonal anti-nectin-3 antibody (Clone 103-A1) (ABIN1105755) specifically detects nectin-3 in IP, IF, IHC (fro), Func and EIA. The antibody is reactive with Mouse samples.

Quick Overview for nectin-3 antibody (Extracellular Domain) (ABIN1105755)

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This nectin-3 antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Enzyme Immunoassay (EIA)

Clone: 103-A1

Product Details anti-nectin-3 Antibody

Binding Specificity: AA 50-400, Extracellular Domain

Specificity: The monoclonal antibody 103-A1 recognizes Mmouse Nectin-3 at aa 50-400.

No Cross-Reactivity: Human, Rat (Rattus)

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Protein G

Immunogen: Recombinant mouse nectin-3 extracellular domain (50-400 aa) fused to IgG Fc

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % Sodium Azide, 0.1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 2-8 °C.

Target Details for nectin-3

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Alternative Name: CD113 / Nectin 3

Background: Nectin-3 is a 83 kDa type I transmembrane glycoprotein. Nectin, originally isolated as poliovirus receptor-related protein (PRR), is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectins are calcium-independent immunoglobulin-like cell-cell adhesion molecules consisting of four members, nectin 1-4. Nectins homophilically and heterophilically trans-interact to form a variety of cell-cell junctions, including cadherin-based adherens junctions in epithelial cells and fibroblasts in culture, synaptic junctions in neurons, and Sertoli cell-spermatid junctions in testis, in cooperation with, or independently of, cadherins. Both nectin-2 and nectin-3 are ubiquitously expressed, whereas nectin-1 is abundantly expressed in brain. Nectin-2 and -3 are expressed in cells where cadherin is not expressed, such as blood cells and spermatids. All members of the nectin family have two or three splice variants. For nectin-3, three isoforms exist: nectin-3α, -3β and -3g of which nectin-3α is the largest. Nectin-3, also known as PRR3, is a transmembrane protein that is predominantly expressed in testis and placental tissues as well in many cell lines. Nectin interacts in vivo with both long and short isoforms of afadin, an actin binding protein, at cadherin-based cell-cell adherence junctions in various tissues and cell lines. Furthermore, the ectodomains of nectin-3 and CD155 (Poliovirus Receptor) have shown strong affinity to each other. Injection of antibody 103-A1 into lumen of seminiferous tubules leads to disruption of the actin filaments in Sertoli cells at the Sertoli-maturing spermatid ectoplasmic specialization and exfoliation of maturing spermatids form the seminiferous epithelium.Synonyms: CDw113, Nectin-3, PRR3, PVRL3, Poliovirus receptor-related protein 3

Gene ID: 58998

NCBI Accession: NP_067470

UniProt: Q9JLB9

Pathways: Cell-Cell Junction Organization