CD14 antibody

Catalog No. ABIN1105870

This Rat Monoclonal antibody specifically detects CD14 in WB, IF and Func. It exhibits reactivity toward Mouse.

Quick Overview for CD14 antibody (ABIN1105870)

Target: CD14

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Functional Studies (Func)

Clone: 14-02-14

Product Details anti-CD14 Antibody

Specificity: The monoclonal antibody Sa14-2 recognizes the mouse monocyte marker CD14.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Protein G Chromatography

Immunogen: CD14 transfectant

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA

Storage: 4 °C

Storage Comment: Store undiluted at 2-8 °C.

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: The CD14 receptor is a pattern recognition molecule in the innate immune response against microorganisms and other exogenous and endogenous stress factors. CD14 was characterized as a receptor for LPS. The CD14 gene consists of two exons which code for a single mRNA that is translated into a protein of 375 amino acids. The CD14 protein is composed of eleven leucin-rich repeats, which are also found in TLR and which are important in PAMP binding. In contrast to TLR, however, CD14 lacks a transmembrane domain, and thus cannot initiate intracellular signal transduction by itself. The most important CD14 signaling co-receptor is toll-like receptor 4 (TLR4), which activates, among others, the nuclear factor κB (NF-κB) inflammatory pathway. The CD14 protein is processed in the endoplasmatic reticulum and expressed as a 55 kDa glycoprotein on the cell surface via a glycosylphosphatidyl (GPI) anchor. Like other GPI-anchored proteins, CD14 accumulates on the cell surface in microdomains known as lipid rafts. CD14 is expressed pre dominantly on the surface of 'myeloid' cells, such as monocytes, macrophages and neutrophils, but at lower levels also on epithelial cells, endothelial cells and fibroblasts. CD14 is also expressed in a soluble form (sCD14). sCD14 may result from secretion of the protein before coupling to the GPI anchor or from shedding or cleavage from the surface of monocytes. sCD14 is present in the circulation and other body fluids and levels of sCD14 in plasma increase during inflammation and infection. CD14 is a molecule with a broad range of functions. In addition to functioning as a pattern recognition receptor for a variety of microbial ligands, CD14 also acts as a receptor for endogenous molecules like intercellular adhesion molecule (ICAM)-3 on the surface of apoptotic cells, amyloid peptid, ceramide, and urate crystals. Ligation of CD14 by these ligands, except for apoptotic cells, mediates activation of inflammatory responses.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

Gene ID: 12475

NCBI Accession: NP_033971

UniProt: P10810

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades