CD40 Ligand antibody

Catalog No. ABIN1105903

Product Details anti-CD40 Ligand Antibody

Target: CD40 Ligand (CD40LG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD40 Ligand antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: This antibody reacts with CD154 antigen.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein A agarose

Immunogen: Human CD154 transfected 300-19 cell (mouse pre-B cell line)

Clone: 5F3

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Prepare a stock solution by dissolving the lyophilized antibody in 100 μL of distilled water. After reconstitution, the IgG concentration should be 1 mg/mL.

Buffer: PBS ( pH 7.2) without preservatives, 1 % sucrose

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store (in aliquots) at -20 °C.

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD154 / CD40L (CD40LG Products)

Synonyms: CD154 antibody, cd40l antibody, CD40L antibody, HIGM1 antibody, IGM antibody, IMD3 antibody, T-BAM antibody, TNFSF5 antibody, TRAP antibody, gp39 antibody, hCD40L antibody, CD40-L antibody, Cd40l antibody, Ly-62 antibody, Ly62 antibody, Tnfsf5 antibody, CD40 ligand antibody, TNF superfamily member 5 antibody, CD40LG antibody, cd40l antibody, Cd40lg antibody

Background: The human CD154 (CD40-ligand) is a 39 kDa type II membrane glycoprotein predominantly expressed on CD4+ T cells, but also on a small proportion of CD8+ cells and NK cells. It belongs to the tumor necrosis factor (TNF) family, while its counter receptor on B cells, CD40, is a member of the TNF receptor/nerve growth factor (NGF) receptor family. Interactions between CD40 on B cells and its ligand on activated T cells results in B cell activation, proliferation, aggregation, and immunoglobulin isotype switching. A defect in the gene encoding CD154 is responsible for X-linked immunoglobulin deficiency with normal or elevated IgM (X-linked hyper IgM). These patients produce IgM but fail to produce downstream Ig isotypes, suggesting that interaction between CD40 on B cells and its ligand on activated T cells plays a critical role in Ig heavy chain switch recombination. The expression of CD154 on activated T cells is tightly regulated. B cells down-regulate the expression of functional CD154 in activated T cells through the inhibition of CD154 mRNA expression and the release of soluble CD40 that binds CD154.Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5

Gene ID: 959

NCBI Accession: NP_000065

UniProt: P29965

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints