CD21 antibody

Catalog No. ABIN1106056

This Mouse Monoclonal antibody specifically detects CD21 in FACS and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for CD21 antibody (ABIN1106056)

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD21 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B-ly4

Product Details anti-CD21 Antibody

Specificity: Clone B-ly4 produces mouse IgG1 immunoglobulins directed against a 145 kDa polypeptide of the C3d receptor. Clone B-ly4 was clustered at the Leucocyte Typing Workshop IV [5].

Purification: Affinity chromatography

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: 0.01 M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2 % BSA, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Fluorochrome labelled product is photosensitive and should be protected from light.

Target Details for CD21

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Alternative Name: CD21

Background: The CD21 antigen is expressed on mature B cells, follicular dendritic cells and pharyngeal and cervical epithelial cells [1,2]. CD21, also known as the Complement receptor type 2 (CR2) or Epstein-Barr virus (EBV) receptor, is a receptor for the C3 activation fragments iC3b and C3d. CD21 is part of a multimeric complex on B cells which includes CD19 and CD81 [3]. The ligand for CD21 is generated during activation of complement by the attachment of C3d to potential antigen. This creates fusion proteins that may cross-link the CD19-CD21-CD81 complex to sIg on antigen-specific B cells. The resulting signal transduction lowers the threshold for cellular activation. This enables an antibody reaction to natural protein antigens containing only one or a few epitopes and therefore unable to effectively cross-link Ig molecules on specific B cells. For example, keyhole limpet hemocyanin (KLH) immune complexes can be taken up and processed by all CR2-bearing B cells, regardless of their antigen specificity. KLH immune complexes taken up this way by non-specific B cells were shown to be presented to KLH-specific, MHC class II Th cells. CD23 may also serve as a ligand for CD21 [3].Synonyms: C3DR, C3d receptor, CR2, Complement C3d receptor, Complement receptor type 2, Dendritic Cell Marker, EBV Receptor, Epstein-Barr virus receptor

Gene ID: 1380

NCBI Accession: NP_001006659

UniProt: P20023

Pathways: Complement System