TLR3 antibody

Catalog No. ABIN1106212

This Mouse Monoclonal antibody specifically detects TLR3 in WB, FACS, IP, IF, IHC (p), IHC (fro) and Func. It exhibits reactivity toward Human, Mouse and Dog.

Quick Overview for TLR3 antibody (ABIN1106212)

Target: TLR3 (Toll-Like Receptor 3 (TLR3))

Reactivity: Human, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: TLR3-7

Product Details anti-TLR3 Antibody

Specificity: The monoclonal antibody TLR3.7 recognizes the 116 kDa human Toll-like receptor 3 (TLR3, CD283).

Cross-Reactivity (Details): Species reactivity (tested):Human, Canine

Purification: Protein G

Immunogen: Human Flag-tagged TLR3 stably expressed by Ba/F3 cells

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % bovine serum albumin

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for TLR3

Target: TLR3 (Toll-Like Receptor 3 (TLR3))

Alternative Name: CD283 / TLR3

Background: Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called 'pathogen-associated molecular patterns'. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NFκB-dependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. TLRs are class I receptors, with a single α-helix that spans the cell membrane. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens, such as lipopolysaccharide (LPS) from the outer membrane of Gram negative bacteria, peptidoglycan fragments from bacterial cell walls and single-stranded and double-stranded RNA from viruses. Some forms of RNA and DNA from pathogens exhibit immutable features that distinguish them from nucleic acids of higher organisms. For example, dsRNA, is a common intermediate of viral replication and a potent indicator of infection. Toll-like receptor 3 (TLR3) recognizes viral double-stranded RNA and its synthetic analog polyriboinosinic:polyribocytidylic acid (poly(I:C)). TLR3 is normally located in acidic endosomes where its luminal ectodomain (ECD) encounters dsRNA and induces type I interferon (IFN), inflammatory cytokine/chemokine production and dendritic cell (DC) maturation via the adaptor protein TICAM-1 (also called TRIF). Based on the different subcellular localization of cytosolic RNA receptors and TLR3, these receptors seem to play distinct roles in anti-viral immune responses.Synonyms: Toll-like receptor 3

Gene ID: 9606

UniProt: O15455

Pathways: TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades