F11R antibody (Extracellular Domain) (FITC)

Catalog No. ABIN1106269

Product Details anti-F11R Antibody (FITC)

Target: F11R (F11 Receptor (F11R))

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This F11R antibody is conjugated to FITC

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The monoclonal antibody BV16 recognizes the human junction adhesion molecule (JAM)-A.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Potein G

Immunogen: Fusion protein consisting of the extracellular domain of human JAM and the Fc portion of human IgGs

Clone: BV16

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % sodium azide, 1 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or or (in aliquots) at -20 °C for longer.

Target Details for F11R

Target: F11R (F11 Receptor (F11R))

Alternative Name: CD321 / JAM1 (F11R Products)

Synonyms: CD321 antibody, JAM antibody, JAM1 antibody, JAMA antibody, JCAM antibody, KAT antibody, PAM-1 antibody, 9130004G24 antibody, AA638916 antibody, ESTM33 antibody, JAM-1 antibody, JAM-A antibody, Jcam antibody, Jcam1 antibody, Ly106 antibody, Jam1 antibody, F11 receptor antibody, F11R antibody, F11r antibody

Background: Together with JAM-C (JAM-2) and JAM-B (VE-JAM or JAM-3), JAM-A belongs to a family of adhesion proteins with a V-C2 immunoglobulin domain organization and their molecular weight is about 30-40 kDa. JAMs are important for a variety of cellular processes, including tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. JAM-A is expressed by endothelial and epithelial cells, platelets, neutrophils, monocytes, lymphocytes and erythrocytes. Like all other JAMs, JAM-A plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-A also plays an important role in leukocyte transmigration. Leukocyte transmigration can be blocked by antibodies and by soluble JAM-A/Fc fusion proteins. Homophilic JAM-A interactions between leukocytes and the endothelium but also heterophilic interactions of JAM-A with the beta2-integrin leukocyte function-associated antigen-1 (LFA-1) are considered to actively guide leukocytes during transmigration. Several studies imply a role for JAM-A in the initiation of atherosclerosis since JAM-A is upregulated on early atherosclerotic endothelium. The adhesion of activated platelets on the activated endothelium is mediated by homophilic interactions of JAM-A.Synonyms: F11R, JAM-A, JCAM, Junctional adhesion molecule 1, Junctional adhesion molecule A, PAM1, Platelet F11 receptor, Platelet adhesion molecule 1

Gene ID: 50848

NCBI Accession: NP_058642

UniProt: Q9Y624

Pathways: Cell-Cell Junction Organization