C5 antibody (N-Term)

Catalog No. ABIN1106795

The Mouse Monoclonal anti-C5 antibody has been validated for Func and ELISA (Detection). It is suitable to detect C5 in samples from Human.

Quick Overview for C5 antibody (N-Term) (ABIN1106795)

Target: C5 (Complement Component 5 (C5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This C5 antibody is un-conjugated

Application: Functional Studies (Func), ELISA (Detection)

Clone: 561

Product Details anti-C5 Antibody

Binding Specificity: N-Term

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein G

Immunogen: Human C5/C5a

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for C5

Target: C5 (Complement Component 5 (C5))

Alternative Name: Complement C5

Background: The complement system is composed of over 30 proteins, activated in response to tissue injury, invading pathogens or other foreign surfaces. The complement pathways can be divided in the activation pathways and lytic pathway. The activation pathways lead via C3 to the cleavage of the fifth complement component C5 into C5a and C5b, resulting in activation of the lytic pahway C5a was first described as a cleavage product of C5 with chemotactic and anaphylatoxic properties. Further characterization revealed that C5a is an essential part of the innate immune response and evidence now suggests that it may also play a role in adaptive immunity. Complement fragment C5a is a 74 residue pro-inflammatory polypeptide. C5a induces smooth muscle contraction, increases vascular permeability, causes degranulation of mast cells and basophils, and release of lysosomal enzymes. In addition C5a stimulates the directed migration of neutrophils, eosinophils, basophils and monocytes. C5a binds to at least two seven-transmembrane domain receptors, C5aR (C5R1, CD88) and C5L2 (gpr77), expressed ubiquitously on a wide variety of cells but particularly on the surface of immune cells like macrophages, neutrophils and T cells. The former is a well-established receptor that initiates G-protein-coupled signaling via mitogen-activated protein kinase pathways, thereby by inducing synthesis of cytokines such as TNF-alpha, IL-1beta, IL-6 and IL-8. Its in vivo blockade greatly reduces inflammatory injury. Much less is known about C5L2, occupancy of which by C5a does not initiate increased intracellular Ca(2+).The widespread expression of C5a receptors throughout the body allows C5a to elicit a broad range of effects. Thus, C5a has been found to be a significant pathogenic driver in a number of immuno-inflammatory diseases. Nowadays C5a is also implicated in non-immunological functions associated with developmental biology, CNS development and neurodegeneration, tissue regeneration, and haematopoiesis.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, CPAMD4, Complement component 5

Gene ID: 9606

UniProt: P01031

Pathways: Complement System, Carbohydrate Homeostasis