Complement Factor B antibody

Catalog No. ABIN1106809

This Mouse Monoclonal antibody specifically detects Complement Factor B in WB, IP and EIA. It exhibits reactivity toward Human.

Quick Overview for Complement Factor B antibody (ABIN1106809)

Target: Complement Factor B (CFB)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Complement Factor B antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: M20-6

Product Details anti-Complement Factor B Antibody

Specificity: This antibody recognizes human complement Factor B/Ba.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Purified

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for Complement Factor B

Target: Complement Factor B (CFB)

Alternative Name: Complement Factor B

Background: Factor B is an acute-phase protein. Levels of factor B increase during inflammation. Complement factor B is a single- chain molecule of 764 amino acids (MW of 90 kD), including a leader peptide of 25 amino acids. Factor B provides the catalytic subunit of the C3/C5 convertases of the alternative complement pathway. Assembly of the C3 convertase (C3bBb) requires binding of factor B to C3b (C3.H2O) and factor D-mediated cleavage of bound factor B resulting in the release of Ba (MW 33 kD). The C3 convertase is stabilized by the binding of properdin. This provides a positive amplification loop for the classical and alternative complement pathways. Bb (MW 60 kD) is the serine protease element of this convertase. After cleavage of C3, the C5 convertase ((C3b)2Bb) is formed. The Bb fragment may be regarded as a better indicator of the alternative pathway of complement activation than Ba as impaired renal filtration does directly influence Ba levels. Whereas Bb is elevated 2.2-fold in chronic renal failure (CRF) patients, plasma concentrations of Ba are 8.4-fold higher in CRF and 16-fold higher in end-stage renal disease (ESRD) patients compared to normals.In addition to complement activation, factor B fragments participate in other immunological functions.Synonyms: BF, BFD, C3/C5 convertase, CFB, Glycine-rich beta glycoprotein, PBF2, Properdin factor B

Gene ID: 629

NCBI Accession: NP_001701

UniProt: P00751

Pathways: Complement System, Proton Transport, Ribonucleoside Biosynthetic Process