TdT antibody (C-Term)

Catalog No. ABIN1107004

This Rabbit Polyclonal antibody specifically detects TdT in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TdT antibody (C-Term) (ABIN1107004)

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TdT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TdT Antibody

Binding Specificity: C-Term

Specificity: This antibody detects DNTT / TDT at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Rat, mouse
Species reactivity (tested):Human

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human TDT .Remarks: Sequence is identical to the related rat and mouse sequence.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for TdT

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Alternative Name: DNTT / TDT

Background: Terminal Deoxynucleotidyl Transferase, also known as TdT and terminal transferase, is a unique DNA polymerase without template direction catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers.1 Its gene is mapped to the region 10q23-q24.2 And TDT Cdna contains an open reading frame of 1,530 basepairs corresponding to a protein containing 510 amino acids.3 TDT may be responsible for inserting nucleotides (N regions) at the V(H)-D and D-J(H) junctions of immunoglobulin genes. The enzyme is present in immature thymocytes, some bone marrow cells,transformed pre-B and pre-T cell lines, and leukemia cells. Additionally, TdT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor.Synonyms: DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal transferase

Gene ID: 1791

NCBI Accession: NP_001017520

UniProt: P04053

Pathways: DNA Damage Repair