Deoxyuridine Triphosphatase (DUT) (Middle Region) antibody

Catalog No. ABIN1107021

This anti- antibody is a Rabbit Polyclonal antibody detecting in WB, IHC (p) and IP. Suitable for Human, Rat and Mouse.

Quick Overview for Deoxyuridine Triphosphatase (DUT) (Middle Region) antibody (ABIN1107021)

Target: Deoxyuridine Triphosphatase (DUT)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details

Binding Specificity: Middle Region

Specificity: This antibody detects DUT in the middle. No cross reactivity with other roteins.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Immunogen affinity purified

Immunogen: Synthetic peptide corresponding to a sequence mapping at the middle region of human dUTPase .Remarks: Sequence is identical to the related rat and ouse sequence.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg himerosal, 0.05 mg NaN3

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details

Target: Deoxyuridine Triphosphatase (DUT)

Alternative Name: DUT

Background: Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) is responsible for maintaining low ntracellular levels of dUTP, thus preventing the incorporation of dUTP into DNA. DUTPase ctivity/expression can be down-regulated using siRNA specifically targeted to dUTPase mRNA and UTPase plays a role in DNA nucleotide metabolism. This protein, present predominantly in the ytoplasm, contains 252 amino acids with a Mr of 26,704. It exhibits 35 % identity with the E. coli UTPase and 53 % identity with the Saccharomyces cerevisiae enzyme. The nuclear and mitochondrial orms of dUTPase are encoded by the same gene with isoform-specific transcripts arising through the se of alternative 5-prime exons. Human dUTPase exhibits 92 % identity with rat. Moreover, this nzyme has profound effects on the efficacy of agents that target thymidylate biosynthesis.Synonyms: Deoxyuridine 5'-triphosphate nucleotidohydrolase mitochondrial, dUTP pyrophosphatase, dUTPase

Gene ID: 1854

NCBI Accession: NP_001020419

UniProt: P33316