eEF1A1 antibody (N-Term)

Catalog No. ABIN1107053

Product Details anti-eEF1A1 Antibody

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Binding Specificity: N-Term

Reactivity: Bacteria

Host: Mouse

Clonality: Monoclonal

Conjugate: This eEF1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: The monoclonal antibody 900 recognizes elongation factor Tu (EF-Tu), a 43 kDa multifunctional protein present in Escherichia coli. The antibody recognizes EF-Tu in organisms belonging to bacterial and archaeal domains, yet no organisms from the eukaryotic domain. The panbacterial distribution of EF-Tu, which is present in large amounts in every prokaryotic cell, renders this protein a good candidate for diagnostic purposes. The highly conserved epitope recognized by monoclonal antibody 900 is located at the very end of the N-terminus of the EF-Tu molecule (SKEKFE).

Cross-Reactivity (Details): Species reactivity (tested):E. coli. Cross react with Bacteroides fragilis, Streptococcus oralis, Bacillus subtilis, Pseudomonas aeruginosa, Burkholderia cepacia, and Deinococcus sp., weakly cross reacts with Mycobacterium tuberculosis.

Purification: Protein G purified

Immunogen: E. coli K-12 C600 Rif ( pKT146) bacteria

Clone: 9E2

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 2-8 °C.

Target Details for eEF1A1

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Alternative Name: EEF1A1 / LENG7 (EEF1A1 Products)

Background: EF-Tu is one of the most abundant proteins present in prokaryotes, representing about 5 % of the total cellular protein of E. coli. During protein biosynthesis, the elongation process, EF-Tu catalyzes the binding of each aminoacyl-tRNA to the ribosome. It also interacts with several macromolecules and guanine nucleotides, including EF-Ts, GDP, GTP, and some ribosomal proteins.Synonyms: CCS-3, CCS3, EEF1A, EF-1-alpha-1, EF-Tu, EF1A, Elongation factor 1 alpha 1, Elongation factor Tu, Eukaryotic elongation factor 1 A-1, FLJ25721, GRAF-1EF, Leukocyte receptor cluster member 7, PTI1, eEF1A-1

Gene ID: 1915

NCBI Accession: NP_001393

UniProt: P68104