Endomucin antibody

Catalog No. ABIN1107088

This Rat Monoclonal antibody specifically detects Endomucin in WB, IHC (fro), IHC (p) and IP. It exhibits reactivity toward Mouse.

Quick Overview for Endomucin antibody (ABIN1107088)

Target: Endomucin (EMCN)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Endomucin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: V-7C7-1

Product Details anti-Endomucin Antibody

Specificity: The monoclonal antibody V.7C7.1 recognizes endomucin, type I membrane protein of 248 amino acids (75 kDa) and shows no signicant homology to any known glycoprotein.

No Cross-Reactivity: Human

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Purified

Immunogen: Mouse endomucin-IgG fusion protein

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % Sodium Azide, 0.1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 2-8 °C.

Target Details for Endomucin

Target: Endomucin (EMCN)

Alternative Name: Endomucin

Background: As a typical mucin-like glycoprotein, endomucin has a high content of serine and threonine residues, suggesting strong O-glycosylation, the sensitivity to O-sialoglycoprotein endopeptidase indicates that endomucin is also a sialomucin. Endomucin is an endothelial-specific sialomucin. It is a constitutively expressed endothelial cell surface protein that is found on all venules but is absent from high endothelial venule cells (HEV) of peripheral and mesenteric lymph nodes as well as Peyer's patches, the specialized site for most efficient lymphocyte trafficking. This could indicate an anti-adhesive function of endomucin, as demonstrated for other sialomucins. Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) is another cell adhesion molecule that contains a mucin-like domain and is expressed on HEV in Peyer's patches, mesenteric lymph nodes and on venules in intestinal lamina propria. In the HEV of mesenteric lymph nodes, the mucin-like domain of a subpopulation of MAdCAM-1 Molecules contains sulfated carbohydrate side chains that interact with L-selectin. The presence of three putative protein kinase C phosphorylation sites in the cytoplasmic tail of endomucin indicates that endomucin has the capacity to be a signaling molecule.Synonyms: EMCN, EMCN2, Endomucin-2, Gastric cancer antigen Ga34, MUC14, Mucin-14

Gene ID: 59308

NCBI Accession: NP_001156994

UniProt: Q9R0H2